1-alpha,25-dihydroxyvitamin D3 Regresses Endometriotic Implants in Rats by Inhibiting Neovascularization and Altering Regulation of Matrix Metalloproteinase

Log in or subscribe to view full content.
Article is also available for purchase the article in one of the available formats.
Basak Yildirim, MD; Tolga Guler, MD; Metin Akbulut, MD; Ozer Oztekin, MD; Gulcin Sariiz, MD

Table of Contents

Postgraduate Medicine:

Volume 126 No. 1


Clinical Features

Purchase this article in one of the formats specified below:

DOI: 10.3810/pgm.2014.01.2730
Abstract: Background:The exact pathogenesis of endometriosis has not been completely discerned. 1-alpha,25-dihydroxyvitamin D3 (1,25[OH][2]D[3]) has been shown to have an anti-angiogenic effect and extracellular matrix-proteases-degrading properties. We hypothesized that 1,25(OH)(2)D(3) may have therapeutic value in the treatment of endometriosis.Methods:Endometrial tissue was implanted into the abdominal peritoneum of 21 Wistar albino rats; the rats were randomized into 3 groups. In Group A (simultaneous group), we simultaneously induced endometriosis and began 1,25(OH)(2)D(3) treatment. Group B rats (sequential group) were treated after endometriosis was documented. Animals in Group C (control group) were followed without any treatment after the development of endometriosis.Results:Histologic score, mean volume, and weight of the explants in Group A and B were found to be significantly lower than those of the control group. Levels of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9) immunoreactivities in Group A and B were also significantly lower compared with Group C. In contrast, intensities of immunoreactivity staining for tissue inhibitor of metalloproteinase-2 (TIMP-2) in Group A and B were significantly higher than that of the control group.Conclusion:1,25(OH)(2)D(3) regresses endometriotic implants in rat models by altering implant levels of VEGF, TIMP-2, and MMP-9.

Keywords: 1-alpha,25-dihydroxyvitamin D3; endometriosis; vascular endothelial growth factor; tissue inhibitor of metalloproteinase-2; metalloproteinase-9